![]() B are not necessarily the ones thought to be physically present in the mass spectrometer, but they more readily convey how to calculate fragment ion molecular weights. Table I, and radical cations are distinguished by a superscript dot (e. In this review, we will use the nomenclature in Fig. Electron capture dissociation spectra exhibit many similarities to ETD spectra however, instruments that produce ETD data are now much more common and will therefore be the focus here. Three processes will be considered here: vibrational excitation via low energy CID electronic excitation from high energy CID and electron transfer dissociation (ETD). Fragment Ions This review only covers de novo sequencing of protonated proteolytic peptides of length less than ∼2. ![]() De Novo Sequencing and Homology Searching.
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